用途:BTC-100X根系生長動態(tài)監(jiān)測系統(tǒng)是利用微根管(Minirhizotron,又稱微根窗)技術(shù)用于非破壞性監(jiān)測分析根系動態(tài)的儀器技術(shù),它是一種非破壞性、定點直接觀察和研究植物根系的方法,其優(yōu)點是在不干擾細根生長過程的前提下,能連續(xù)監(jiān)測單個細根從出生到死亡的變化過程,也能記錄細根乃至根毛和菌根的生長、生產(chǎn)和物候等特征,是估計生態(tài)系統(tǒng)地下C分配和N平衡研究的有效方法,結(jié)合所提供根系分析軟件,能夠?qū)⒏迪嚓P(guān)數(shù)據(jù)定量化,包括根的長度、面積、根尖數(shù)量、直徑分布格局、死亡根及存活根數(shù)量等等。還可以根據(jù)用戶需求監(jiān)測土壤水分狀況,從而研究根系所在區(qū)域內(nèi)溶質(zhì)運移及水分脅迫所引起的生理變化,廣泛運用于苗木培養(yǎng)、作物生長模型研究、根系病理分析、昆蟲行為生態(tài)等領(lǐng)域。
工作原理:BTC-100X根系生長動態(tài)監(jiān)測系統(tǒng)利用微根管技術(shù),整套系統(tǒng)由成像頭、控制模塊、手柄、光源、微根管等部件組成。將成像頭伸入埋設(shè)在根系周圍的微根管內(nèi),通過控制模塊進行根系圖像抓取成像,然后使用預(yù)裝在電腦上的專業(yè)根系分析軟件系統(tǒng)對混合圖像進行分析,從而跟蹤了解其生長過程。
基本組成 控制模塊
手柄 帶光源的成像頭
分析軟件
技術(shù)參數(shù):
監(jiān)測分析參數(shù) | 細根長、細根直徑、細根面積、細根總長、細根總面積、細根平均直徑、細根數(shù)量及生物量、細根壽命、細根周轉(zhuǎn)率等,其100倍高倍放大功能,可用于監(jiān)測分析根毛及菌根生理生態(tài)和動態(tài)。 |
成像頭 | NTSC制式彩色成像頭(可選PAL制式),防水性能設(shè)計,高分辨率,帶白光光源。每個視頻幀看到管壁的面積為長12.5毫米×寬18毫米。 |
放大功能 | 100倍 |
光源 | 標準白光光源,可選紫外光源,以幫助識別活的細根或新萌發(fā)的根,或?qū)晒鈽擞涍M行識別成像。 |
控制模塊功能 | 控制系統(tǒng)含電源開關(guān),控制成像頭的光學(xué)放大縮小開關(guān),紫外光源的開關(guān),成像焦距的微調(diào)開關(guān)。 |
手柄 | 1.2~2.2米伸縮式手柄 |
供電 | 12V可充電電池,可連續(xù)工作約8小時。 |
連接電纜長度 | 4.8米 |
微根管尺寸 | 直徑51毫米×長度1.8米,可定制其他長度觀測管。 |
應(yīng)用文獻:
1. 白文明、程維信、李凌浩,微根窗技術(shù)及其在植物根系研究中的應(yīng)用。生態(tài)學(xué)報,2005,25(11):3076-3081.
2. 李俊英、王孟本、史建偉,應(yīng)用微根管法測定細根指標方法評述。生態(tài)學(xué)雜志,2007,26(11):1842-1848.
3. 邱俊、谷加存、姜紅英等,樟子松人工林細根壽命估計及影響因子研究。植物生態(tài)學(xué)報,2010,34(9):1066-1074.
4. 宋森、谷加存、全先奎等,水曲柳和興安落葉松人工林細根分解研究。植物生態(tài)學(xué)報,2008,32(6):1227-1237.
5. 于水強、王政權(quán)、史建偉等,氮肥對水曲柳和落葉松細根壽命的影響。應(yīng)用生態(tài)學(xué)報,2009,20(10):2332-2338.
6. A.L.Kalyn, K.C.J.Van Rees. Contribution of fine roots to ecosystem biomass and net primary production in black spruce, aspen, and jack pine forests in Saskatchewan. Agricultural and Forest Meteorology, 2006, 140:236-243.
7. C. E. Wells, D. M. Glenn, and D. M. Eissenstat. Soil insects alter fine root demography in peach(prunus persica). Plant, Cell and Environment, 2002, 25: 431-439.
8. Carolyn S. Wilcox, Joseph W. Ferguson, George C.J. Fernandez, etc. Fine root growth dynamics of four Mojave Desert shrubs as related to soil moisture and microsite. Journal of Arid Environments, 2004, 56:129-148.
9. Christel C.Kern, Alexander L. Friend, Jane M.Johnson, etc. Fine root dynamics in a developing Populus deltoides plantation. Tree Physiology, 2004, 24:651-660.
10. Colleen M. Iversen, Joanne Ledford and Richard J. Norby. CO2 enrichment increases carbon and nitrogen input from fine roots in a deciduous forest. New Phytologist, 2008, 179: 837-847.
11. D.G.Milchunas, J.A.Morgan, A.R.Mosiers, etc. Root dynamics and demography in shortgrass steppe under elevated CO2, and comments on minirhizotron methodology. Glogal Change Biology, 2005, 11:1837-1855.
12. James F.Cahill Jr., Gordon G. McNickle, Joshua J.Haag, etc. Plant Integrate Information about Nutrients and Neighbors. Science, 2010, 328: 1657.
13. Jinmin Fu and Peter H. Dernoeden. Creeping Bentgrass Putting Green Turf Responses to Two Summer Irrigation Practices: Rooting and Soil Temperature. Crop Scinece, 2009, Vol. 49: 1063-1070.
14. John S. King, Timothy J. Albaugh, H. Lee Allen, etc. Below-ground carbon input to soil is controlled by nutrient availability and fine root dynamics in loblolly pine. New Phytologist, 2002, 154: 389-398.
15. Laurent Misson, Alexander Gershenson, Jianwu Tang, etc. Influences of canopy photosynthesis and summer rain pulses on root dynamics and soil respiration in a young ponderosa pine forest. Tree Physilogy, 2006, 26:833-844.
16. Michael F. Allen. Mycorrhizal Fungi: Highways for Water and Nutrients in Arid Soils. Vadose Zone Journal, 2007, 6(2): 291-297
17. Seth G. Pritchard, Hugo H. Rogers, Micheal A Davis etc. The influence of elevated atmospheric CO2 on fine root dynamics in an intact temperate forest. Global Change Biology, 2001, 7: 829-837.
18. Weixin Cheng, David C.Coleman and James E.Box Jr. Measuring root turnover using the minirhizotron technique. Agriculture, Ecosystems and Environment, 1991, 34:261-267.